Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1.

نویسندگان

  • Takahiro Inoue
  • Mami Ishikawa
  • Yu Sumiya
  • Haruka Kohda
  • Toshio Inui
  • Daisuke Kuchiike
  • Kentaro Kubo
  • Yoshihiro Uto
  • Takahito Nishikata
چکیده

BACKGROUND As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. MATERIALS AND METHODS Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. RESULTS Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. CONCLUSION Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.

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عنوان ژورنال:
  • Anticancer research

دوره 35 8  شماره 

صفحات  -

تاریخ انتشار 2015